Zebrafish Research
Zebrafish Cognitive Learning Interface (ZebCLI):
My project is focused on developing the Zebrafish Cognitive Learning Interface (ZebCLI), a system designed to study zebrafish conditional learning capabilities. We can deliver controlled stimuli and track their behavior to better understand learning, memory, and cognitive functions. This project aims to improve how we study the brain and its responses, with potential applications in drug testing and developing treatments for neurological disorders.
Slides: Powerpoint Slide
Proposal: Project proposal
Protocol: Will be published after publication
Codes: Will be published after publication
Skills: Python, Microcontroller, 3D Printing, Drug Screening, Circuits Design, Machine Learning
Timeline: February 2024 - Present (in progress)
Updates:
Apr 2024 Installed the LED Matrix Panel in 3D Model
May 2024 Implemented the codes for visual stimulus
May Jun 2024 3D Printing of the arena
Jul Aug 2024 Electroshock generation system
G-force Tolerance of Zebrafish Over Time
G-force is a measure of acceleration. Human can only tolerate a maximum of 6 G within a minute but zebrafish can withstand thousands times higher gravitational forces than human. To advance our understanding of biological responses to extreme physical conditions, our team systematically applied varying levels of centrifugal force to assess the survival rates of embryos at different developmental stages. After this, we did some drug screening to find out how the mechanism behind works.
Protocol: Will be published after publication
Skills: qPCR, Western Blotting, Protein purification
Timeline: July 2023 - Present (still in progress)
Updates:
March 2024 Survival Curve
April to Present Drug screening & Compound testing
Using lightsheet and confocal for brain imaging
To advance the application of adult zebrafish in research, we developed a three-dimensional digital atlas of the adult zebrafish brain. This comprehensive tool was constructed through a combination of tissue clearing techniques, light-sheet fluorescence microscopy, and antibody staining. The resulting images facilitated the detailed segmentation of the atlas into hundreds of distinct regions, encompassing the entire adult zebrafish brain.
Skills: Protein fixation, Depigmentation, Fluorescence Microscopy
Time: Summer 2023
Other Engineering Research & Project
Bone Phantom Design for Med Students:
Our team designed a bone phantom that mimics the structure of a femur to help medical students practice surgical drilling. This low-cost, easily reproducible model simulates real bone, allowing students to improve their skills without relying on limited real bones, ultimately reducing errors in surgery.
Documents: Final Product
Skills: 3D printing, Solid mechanics, COMSOL Multiphysics
Time: Winter 2023
Other independent study / small project/ team work:
May 2023 Team work: Amplifier and Filter in circuit design
(learn more)
April 2023 Team work: Sounds Signal Analysis
(learn more)